embryo Freezing & Thawing


 

CE Marked (CE 0086)

NOT AVAILABLE IN US

Embryo Freezing: For equilibrium (slow) freezing of human embryos between 2PN and 4 cell stage

Embryo Freezing Kits
LGEF-310, 3 x 10 ml
LGEF-120, 12 x 10 ml

Embryo Thawing Kits
LGET-310, 3 x 10 ml (1 X T1; 1 X T2; 1 X T3)
LGET-410, 12 x 10 ml (4 X T1; 4 X T2; 4 X T3)

Embryo Freezing & Thawing Kits
LGFT-310, 3 x 10 ml (3 X LGEF-010; 3 X T1; 3 X T2; 3 X T3)

Indications for Use  

Embryo Freezing: For freezing human embryos between 2PN and 4 cell stage.
Embryo Thawing: For thawing human embryos between 2PN and 4 cell stage.

     
Storage and Shelf Life   Store at 2-8°C and protected from light. Twelve (12) months from the date of production when stored unopened at 2–8°C and protected from light. Do not freeze before use. Keep away from light. Discard unused medium within 7 days after opening. Do not use after expiry date.
     
Disposal Consideration   Treat or dispose of waste material in accordance with all local state/provincial, and national requirements. Dispose with laboratory waste.
     
Composition  

Embryo Freezing & Thawing are propanediol based cryopreservation media which also contain PBS, sucrose and human serum albumin (14-15 mg/ml).

     
Recommendations for Use  

For a full and detailed recommended protocol of use for Embryo Freezing & Thawing please contact us at customerservice@lifeglobal.com.

     
Quality Control Specifications  
Paramater Specification
pH 7.2-7.4
Osmolality – Embryo Thaw 3 only 530-560 mOsM
Endotoxin (LAL) < 0.25 EU/ml
Sterility Test (bacterial and fungal screen, SAL 10–3) PASS
1-cell Mouse Embryo Assay – Embryo Thaw 3 only
(% expanded blastocysts at 96 h of culture)
> 80%
     
Precautions and Warnings  

Embryo Freezing & Thawing contains small amounts of Human Serum Albumin (HSA). The Human Serum Albumin used in the preparation of this product has been heated at 60°C for ten hours.

Caution: Standard measures to prevent infections resulting from the use of medicinal products prepared from human blood or plasma include selection of donors, screening of individual donations and plasma pools for specific markers of infection and the inclusion of effective manufacturing steps for the inactivation/removal of viruses. Despite this, when medicinal products prepared from human blood or plasma are administered, the possibility of transmitting infective agents cannot be totally excluded. This also applies to unknown or emerging viruses and other pathogens. There are no reports of virus transmissions with albumin manufactured to European Pharmacopoeia specifications by established processes. It is strongly recommended that every time that Embryo Freezing & Thawing is administered to a patient, the name and batch number of the product are recorded in order to maintain a link between the patient and the batch of the product.

Always wear protective clothing when handling specimens.

Always work under strict hygienic conditions (e.g. LAF-bench ISO Class 5) to avoid possible contamination.

Only for the intended use. Do not resterilize.